Separation and identification of (Z)-and (E)-diastereomers of resveratrol and piceid in Polygonum cuspidatum by microemulsion TLC
- VernacularTitle:微乳薄层色谱法鉴别虎杖中白藜芦醇和虎杖苷的顺、反异构体
- Author:
Yu ZHANG
;
Shuhong YU
;
Qian LI
;
Lijie GUO
;
Jing WANG
;
Jianpeng ZHA
- Publication Type:Journal Article
- Keywords:
resveratrol;
piceid;
diastereomers;
microemulsion;
polyamide film;
thin layer chromatography;
Polygonum cuspidatum
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To investigate the application of microemulsion TLC to separation and identification of(Z)-and(E)-resveratrol and piceid in Polygonum cuspidatum based on polyamide film as stationary phase.METH-ODS: SDS-n-butane-n-heptane-aqueous microemulsion as the development in combination with aminic acid and methonal as the modifier,the influence of the change in components of microemulsion on the resolution was exam-ined to search for the optimal separation condition of Polygonm cuspidatum extract collected from 6 production places.RESULTS: The best mobile phase was the microemulsion containing 4% SDS,80% water-methyl acid-methanol(1.0 ∶ 2.0 ∶ 1.0).As compared with conventional TLC,resolution of microemulsion TLC for resveratrol and piceid were improved markedly.CONCLUSION: Microemulsion TLC is an efficient,simple and new method for separating and identifing(Z)-and(E)-diastereomers of resveratrol and piceid in Polygonum cuspidatum.
