Determination of peoniflorin,ferulic acid and icariin in Bushen Huoxue Granule
- VernacularTitle:HPLC测定补肾活血颗粒中芍药苷,阿魏酸,淫羊藿苷的含量
- Author:
Ming KONG
;
Linying ZHU
;
Peijuan WANG
;
Yunru PENG
- Publication Type:Journal Article
- Keywords:
Bushen Huoxue Granule;
peoniflorin;
ferulic acid;
icariin;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method of determining peoniflorin,ferulic acid and icariin in Bushen Huoxue Granule(Herba Epimedii,Radix Angelicae Sinensis,Radix Paeoniae Alba,etc.).METHODS: HPLC-DAD was used to determine peoniflorin,ferulic acid and icariin.The samples were separated on Alltima C18(5 ?m,250 mm ? 4.6 mm) with mobile phase consisted of methanol-0.1% phosphoric acid(gradient elution).RESULTS: The average recoveries of peoniflorin,ferulic acid and icariin were 96.7%,97.5%,99.6% and RSD were 1.37%,3.13%,0.96%.respectively.The peoniflorin,ferulic acid and icariin samples showed a good relationship in the ranges of 0.318-3.970 ?g,0.096-1.200 ?g,0.414-5.175 ?g in the chosen HPLC condition.CONCLUSION: The method is simple,accurate and reproducible and can be used for the quality control of granule.
