HPLC fingerprints of Platycodon grandiflorum from different habitats and the determination of platycodin-D
- VernacularTitle:不同产地桔梗药材HPLC指纹图谱及桔梗皂苷D含量测定研究
- Author:
Xifeng LI
;
Yunfeng DU
;
Xinnian XIE
;
Sumei LIU
;
Zhe HAO
- Publication Type:Journal Article
- Keywords:
Platycodon grandiflorum;
fingerprint;
platycodin-D;
determination
- From:
Chinese Traditional Patent Medicine
1992;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish HPLC-fingerprints and quantitatively determine platycodin-D from Platycodon grandiflorum.METHODS: HPLC analysis was carried out on Hypersil C18 column(250 mm ? 4.6 mm,5 ?m),with a mobile phase of acetonitrile-0.05 mol/L phosphoric acid system,gradient elution,with a flow at 0.5 mL/ min,an ultraviolet detection wavelength was at 210 nm for fingerprint and at 206 nm for platycodin-D,column tem-perature at 30 ℃.RESULTS: Twelve common peaks were identified in chromatograms with reference to platycod-in-D peak from the 18 batches of the samples.CONCLUSION: The method of the HPLC-fingerprint and quantita-tive analysis is rapid,simple and accurate with a good reproducibility and can be used for the quality control of Platycodon grandiflorum.
