Effect on Pathological Changes and TGF-?_1 in Bronchopulmonary Tissue in Asthmatic Rat Model Treated by Herbs of Dispersing Lung Qi and Invigorating Spleen Qi
- VernacularTitle:宣肺健脾中药对哮喘大鼠支气管-肺组织病理学和转化生长因子-?_1的影响
- Author:
Zuoying MA
;
Shan HE
;
Weiling YUAN
- Publication Type:Journal Article
- Keywords:
herbs of dispersing lung Qi and invigorating spleen Qi;
Xiaozhuping;
asthma;
airway remodeling;
TGF-?1;
rat
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of bronchial asthma in rats treated by Chuanzhuping,a prescription which has the function of dispersing lung Qi and invigorating spleen Qi,and explore its possible mechanism.Method Thirty-two male Wistar rats of SPF level were divided into four groups randomly:control group(A),asthma group(B),Dexamethasone group(C) and Xiaozhuping group(D).Except group A,the others were established animal model of asthma.After drawing materials,hematoxylin and eosin(HE) staining were performed.The pathological changes in bronchopulmonary tissue of rats were detected.The basementmembrane perimeter(Pbm),the airwaywall area(Wat) and the smooth musclewall area(Wam) were measured and calculated by using image analysis system.The concentration of transforming growth factor-?1(TGF-?1) in bronchopulmonary tissue was observed by immunohistochemical method and the integral optical density(IOD values) of it was measured.Results In bronchopulmonary tissue of group B,Wat,Wam and the concentration of TGF-?1 increased,and were significantly different from that in group A(P0.05).In group D,the concentration of TGF-?1 decreased and was significantly different from that in group C(P
