Determination of Chlorogenic Acid in Shuanghuanglian Keli by HPLC
- VernacularTitle:双黄连颗粒中绿原酸含量的HPLC测定
- Author:
Hongtao ZHANG
;
Junan CAI
;
Xinhui GUO
- Publication Type:Journal Article
- Keywords:
Shuanghuanglian Keli;
chlorogenic acid;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method for detemining the content of chlorogenic acid in Shuanghuanglian Keli by HPLC.Methods Using Diamonsil ODS1 C18 column,with methanol-water-glacial acetic acid(15:85:1) as the mobile phase,detection wavelength as 324 nm and flow rate was 1.0 mL/min.Results The calibration curve was linear at the range of 0.060～1.210 ?g for chlorogenic acid and the equation was Y=105 427X+586.43,r2=0.999 5.The average recovery was 99.3% and RSD was 0.82%(n=6).Conclusion This method was simple,accurate and proper,and the reduplication of the result was good,which provide scientific quantitative analysis method of chlorogenic acid in Shuanghuanglian Keli.
