Determination of Capsaicin and Dihydrocapsaicin in General Alkaloids of Capsaicin by HPLC
- VernacularTitle:HPLC法测定辣椒精中辣椒碱和二氢辣椒碱的含量
- Author:
Bosheng SHI
;
Lihong ZU
- Publication Type:Journal Article
- Keywords:
general alkaloids of Capsaicin;
capsaicin;
dihydrocapsaicin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method to determine the two principal constituent-capsaicin and dihydrocapsaicin in general alkaloids of Capsaicin.Method The analysis was performed on C18 column(4.6 mm?250 mm,5 ?m),with methanol-water(70:30) as the mobile phase.The flow rate was 1.0 mL/min and the detection wavelength was set at 280 nm.Result The linear relationships of capsaicin and dihydrocapsaicin were within the range of 1.955～7.820 ?g(r=0.999 9) and 2.709～7.224 ?g(r= 0.995 5),respectively.The average recoveries were 101.57% and 108.87%,respectively.Conclusion The method is convenient,the result is accurate and reliable,and can be used to determine the content of two principal constituent in general alkaloids of Capsaicin.
