Determination of ?-sitosterol in Rhizoma Heterosmilacis Japonicae by HPLC
- VernacularTitle:HPLC法测定白土茯苓中?-谷甾醇的含量
- Author:
Wenxing ZHI
;
Yuchuan CHENG
;
Xianrong LI
- Publication Type:Journal Article
- Keywords:
?-sitosterol;
Rhizoma Heterosmilacis Japonicae;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish the method to determine the content of ?-sitosterol in Rhizoma Heterosmilacis Japonicae.Method ?-sitosterol in Rhizoma Heterosmilacis Japonicae was determined by HPLC.Chromatographic column was sinachrom ODS-BP 5 ?m column(4.6 mm?250 mm).Mobile phase was methanol-0.1% H3PO4 liquor.Detection wavelength was 210 nm.Flow rate was 1.0 mL/min.Result ?-sitosterol showed a good linear curve in the range of 0.22～8.8 ?g(r=1.000 0).The average recovery was 96.5% with RSD of 1.15%.Conclusion The method was accurate and reproducible,and can be used to control the quality of Rhizoma Heterosmilacis Japonicae.
