Inhibition of humulon on arylamine N-acetyltransferase-1 activity and gene expression in SGC-7901 cells
- VernacularTitle:葎草酮对人胃癌细胞SGC-7901 N-乙酰基转移酶1活性及基因表达的抑制作用
- Author:
Shiyong GAO
;
Lang LANG
;
Xiang ZOU
;
Chenfeng JI
;
Yubin JI
;
Qiang MA
;
Lei YUE
;
Zhongyuan QU
;
Ming SHANG
- Publication Type:Journal Article
- Keywords:
humulon;
SGC-7901 cell;
N-acetyltransferase-1(NAT1);
NAT1 mRNA expression
- From:
Chinese Traditional and Herbal Drugs
1994;0(05):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the anti-tumor effects of humulon on arylamine N-acetyltransferase-1(NAT1)activity.Methods Employing HPLC,using PABA as substrate,in intact SGC-7901 cells and their cytoplasm,making PABA being acetylated to Ac-PABA by NAT1 as the activity of NAT1.Reverse transcriptase polymerase chain reaction(RT-PCR)assay was used to study the expression of the NAT1 mRNA.Results The results show that humulon could inhibit the production of Ac-PABA in intact SGC-7901 cell and the cytoplasm,the production of Ac-PABA was gradually increased with the interaction time increasing.But comparing with corresponding negative control group's,the production of Ac-PABA was decreased evidently and the humulone could inhibit the expression of NAT1 mRNA.Conclusion Humulon could prevent the occurrence and deterioration of cancer.Its mechanisms can be attributed to its effect on decreasing the production of acetylation of carcinogenic aromatic amines,which is acetylated from aromatic amines,and inhibiting the NAT1 activity and expression of NAT1 mRNA.
