A study and evaluation of bacteriophage's utilization in rapid detection of Mycobacterium tuberculosis
- VernacularTitle:结核分枝杆菌噬菌体试剂盒的研制与初步评价
- Author:
Li PENG
- Publication Type:Journal Article
- Keywords:
Bacteriophage(phage);
Mycobacterium tuberculosis;
Diagnosis
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To study and evaluate the utilization of bacteriophage in rapid detection of Mycobacterium tuberculosis in clinical samples.Methods:The titer of replicated phage with different methods in 24 or 48 hours were compared,including centrifuge-filter method and incubate-filter method with 0.2?m filter.When the phages were preserved at different conditions with various preservers,the titer was tested and compared in 28 weeks.Other agents of the method,including virucide and help bacteria(Mycobacterium smegmatis) were investigated at different time when they were preserved at 4℃.After the phage assay was established,34 sputum samples were tested by both phage assay and culture method in L-J medium.Based on the results of culture method,phage assay in detection of mycobacterium tuberculosis were evaluated by the sensitivity,specificity and accordance.Results:①The titer of replicated phage was high,when it had been collected after infected for 48 hours.The collection through incubation-filter method was simple and effect.② The phage stored at 4℃ with liquid form was stable,and the declined titer was not more than 101.when the preserver was phage's host bacteria and it was stored at 4℃,it was as stable as that in dehydration form.③The important agents,including virucide and help bacteria,had the same character in 40 weeks,when they were stored at 4℃.④ Compared with the results of culture method,the sensitivity,specificity and accordance of phage assay in rapid detection of mycobacterium tuberculosis were 86.2%,66.7%,and 84.4% respectively.Conclusion:The phage assay could detect viable Mycobacterium tuberculosis of sputum samples in 48 hours.it is feasible to produce testing reagent box from the phage,and can be expected to be used for clinical.
