Enhanced human MxA gene expression in mice liver by ultrasound and microbubble destruction technique
- VernacularTitle:超声微泡破碎技术促进人MxA基因在小鼠肝脏转染的实验研究
- Author:
Aiping LIU
- Publication Type:Journal Article
- Keywords:
Ultrasound contrast agent;
Ultrasound;
Gene transfection;
Irradiation parameter
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate whether expression of MxA gene could be enhanced by intravenous injection of gene and contrast agent with ultrasound wave irradiation in mice liver and to investigate the effects of different ultrasound parameters on gene expression.Methods:(1) Effect of different methods on transfection efficiency:24 Kunming mice were randomly divided into 4 groups as plasmid group,plasmid and microbubble group,ultrasound and plasmid group,and plasmid,ultrasound and microbubble group.After 7 days,the lungs,hearts,spleens,kidneys,lungs and skeletal muscles were harvested and half quantization of MxA protein was detected with immunofluorescence technique.(2) Effects of different parameters on gene expression:20 Kunming mice were randomly divided into 4 groups as 0.25 W/cm2 group,0.5 W/cm2 group,0.75 W/cm2 group and 1.0 W/cm2 group according to different ultrasound parameters.After 7 days,liver tissues were harvested for the half quantization of MxA by the same method described above.Results:(1) Number of liver cells with positive expression was the highest in plasmid,ultrasound and microbubble group,followed by ultrasound and plasmid group,plasmid and microbubble group,and plasmid group in order;There was significant statistical difference between either two groups(P
