Effects of antisense VEGF-C gene on vegetation process of implanted gastric tumor SGC-7901 in nude mice
- VernacularTitle:胃癌裸鼠移植瘤生长过程中反义血管内皮生长因子-C的作用
- Author:
Peng ZHU
- Publication Type:Journal Article
- Keywords:
Nude mice;
Transplantable tumor;
Antisense vascular endothelial growth factor-C;
Tumorigenicity;
Vascular generation
- From:
Journal of Chongqing Medical University
1986;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effects of tumorigenicity and vascular generation of nude mice on antisence vascular endothelial growth factor-C(antisense VEGF-C) gene transinfection of human gastric carcinoma cell by observing the vegetation process of implanted gastric tumor.Methods:30 nude mice were divided into three groups randomly:transgenic anti-sense VEGF-C group,transgenic pure liposome group and non-transgenic group.The cell suspension of gastric carcinoma cells SGC-7901 non-transinfected or transinfected by anti-sense VEGF-C gene or pure liposome were subcutaneously injected into those mice.After injection,the size and growth velocity of those neoplasma were detected.The Microlymphatic vessel density(MLVD) and microvessel density(MVD) of tumor were detected too.Results:The neoplasma grew more slowly and was smaller in the mice in those transinfected by anti-sense VEGF-C gene than in non-transgenic ones(P0.05).Conclusions:The antisense VEGF-C gene suppress the tumorigenicity and lymphangiogenesis in the human SGC-7901 gastric carcinoma cell apparently.but it had little effects on angiogenesis of the tumor.VEGF-C might participate the lymphangiogenesis of the gastric tumor.
