Determination of Kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa Stearn by HPLC
- VernacularTitle:HPLC法测定蒙药玉簪花中山奈酚葡萄糖苷的含量
- Author:
Jian HE
;
Ying GAO
;
Weimin LI
- Publication Type:Journal Article
- Keywords:
Hosta ventricosa stearn;
Kaempferol-7-O-?-D-glucopyranoside;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a HPLC method for the determination of kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa stearn.Methods HPLC ana1ysis was carried out on a Phenomenex Luna C18(250 mm?4.6 mm,5 ?m) column,gradient elution with the mobile phase of 0.5 % phosphate acid in water-methanol.The detective wavelength was set at 364 nm.Results calibration curve was linear within the range of 0.113 ～ 2.260 ?g for kaempferol-7-O-?-D-glucopyranoside,coefficient correlation being 1.The average recovery was 101.1 %(RSD= 2.58 %,n=6).Conclusion The content of kaempferol-7-O-?-D-glucopyranoside in Hosta ventricosa stearn is 0.2 mg/g(0.02 %) on average.This method is simple,accurate and practical for the determination of kaempferol-7O-?-D-glucopyranoside.
