Determination of [6]-Gingerol in Rat Plasma by HPLC-UV
- VernacularTitle:HPLC-UV法测定大鼠血中[6]-姜酚的浓度
- Author:
Suzhen JIANG
;
Suiqing MI
;
Ningsheng WANG
- Publication Type:Journal Article
- Keywords:
[6]-gingerol;
Gingerols;
HPLC-UV;
Plasma concentration
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To develop a HPLC-UV method for the determination of [6]-gingerol in the rat plasma.Methods [6]-Gingerol was extracted from the rat plasma by using liquid-liquid extraction,then was separated on a Hypersil C18 column(250 mm?4.6 mm,5 ?m).The mobile phase was acetonitrile and water(45:55,v/v) with a flow-rate of 1.0 mL/min.The eluate from the HPLC column was monitored by the spectrophotometric detector at 280 nm.The injection volume was 20 ?L.[6]-Gingerol was identified based on its retention time compared with the reference standard.Results The linear calibration curve was obtained in the concentration range of 0.25～5.0 ?g/mL.The low quantitative limit was 0.1 ?g /mL.The RSD of inter-day was
