Optimized formulation of alkanin liposome by a combination of orthogonal design with central composite design/response surface method
- VernacularTitle:正交实验联用星点设计-效应面法优化紫草素脂质体处方
- Author:
Jianguo XING
;
Xinchun WANG
;
Yongjun ZHANG
;
Yong YUAN
;
Yuhong PU
- Publication Type:Journal Article
- Keywords:
alkanin;
liposome;
orthogonal design;
central composite design
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To select an optiomal formulation of liposome entrapped alkanin prepared with ethanol injection in the combination of orthogonal design and central composite design/Response surface method.METHODS:Five influencing factors,including ratio of alkanin to liposome and soyabean lecithin to cholesterol,volume ratio of ethanol phase to water phase,soybean concentration and mannitol concentration were chosen as indices by orthogonal design.Then the method utilized the central composite design,which fitted multivariate linear and second/third order nonlinear regression equation,to find out the optimal formulation.RESULTS:Third order regression equation gained made the good prediction about entrapment(85.4%) and alkanin load(5.16%),and exhibited no significant difference between theoretic value and measured value.CONCLUSION:The combination of orthogonal design with central composite design is a feasible and convenient method to optimize the prescription of alkanin-lipo-some.
