Determination of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule by HPLC
- VernacularTitle:HPLC同时测定消栓通络胶囊中阿魏酸、芦丁和丹酚酸B的含量
- Author:
Zhimao DU
;
Anjun HUANG
;
Juan BAI
- Publication Type:Journal Article
- Keywords:
Xiaoshuan Tongluo Capsule;
ferulic acid;
rutin;
salvianolic acid B;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a method for determining the contents of ferulic acid,rutin and salvianolic acid B in Xiaoshuan Tongluo Capsule(Rhizoma Chuanxiong,Radix et Rhizoma Salviale miltiorrhizale,Radix Astragali,Radix et Rhizoma notoginseng,Flos Sophorale,etc.) METHODS:The column was Linksil C18(250mm?4.6 mm,5 ?m).The mobil phase was methanol-acetonitrile -0.1% acetic acid(25:5 :70).The detection wave-lenght was set at 320 nm.The flow rate was 1.0 mL/min.RESULTS:The calibration curves of ferulic acid,rutin and salvianolic acid B were in good linearity over the ranges of 0.004-0.08 ?g,0.432-8.64 ?g,0.088-1.76 ?g,respectively(r=0.999 9).And average recoveries(n=5) were 98.9 %,100.1 % and 98.2 %,respectively and RSD were 1.1 %,1.5 %,1.4 %.CONCLUSION:The method is simple,rapid,reliable and can be used for the quality control of Xiaoshuan Tongluo Capsule.
