Molecular Identification of Amomum villosum Lour.Based on Sequence Analysis of 26S rDNA D1-D3 Region and matK Gene
- VernacularTitle:基于26S rDNA D1-D3区和mat K基因序列分析的阳春砂分子鉴别
- Author:
Qionglin HUANG
;
Jinfen YANG
;
Zhonggang DUAN
;
Rui HE
;
Ruoting ZHAN
;
Honghua XU
;
Hui XU
;
Weiwen CHEN
- Publication Type:Journal Article
- Keywords:
AMOMUM VILLOSUM LOUR.;
SEQUENCE ANALYSIS;
MOLECULAR IDENTIFICATION
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a molecular identification method for three cultivars of Amomum villosum Lour.(AVL),thus to provide scientific evidence for the identification,selection and breeding of AVL.Methods The fragments of 26S rDNA D1-D3 region and matK gene of three cultivars of AVL and Amomum longiligulare T.L.Wu were amplified by polymerase chain reaction(PCR) and with corresponding primers,and then their sequences were analyzed,and phylogenetic tree was constructed based on the sequences.Results We obtained 739 bp in 26S rDNA D1-D3 sequence.Differences in 4 basic sites of 739 bp were shown between AVL and Amomum longiligulare T.L.Wu.The two cultivars of AVL,Changguo and Yuanguo,had the same sequence,but there was a difference in one basic site of Changguo and Yuanguo from Chunxuan.The phylogenetic tree based on 26S rDNA D1-D3 sequence revealed the difference between Chunxuan and the other two cultivars of AVL.We also obtained 824 bp in matK gene sequence.The three cultivars of AVL showed the consistent sequence,but there was a difference in one basic site of three cultivars of AVL from Amomum longiligulare T.L.Wu.Conclusion We can identify the three cultivars of AVL through the sequence differences at the molecular level,and Chunxuan has a closer genetic relationship with Amomum longiligulare T.L.Wu.
