Study on the In-vitro Microdialysis Recovery of Nicotine and Its Influence Factors
- VernacularTitle:烟碱微透析回收率的体外测定方法及影响因素研究
- Author:
Xiang CHEN
;
Jiajun LING
;
Liang CHEN
- Publication Type:Journal Article
- Keywords:
NICOTINE/analysis;
MIERODIALYSIS;
CHROMATOGRAPHY,HIGH PRESSURE LIQUID
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
1999;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To detect the in-vitro microdialysis recovery of nicotine by decrement method(i.e.retrodialysis) and to investigate its influence factors,thus to supply experimental evidence for the in-vivo microdialysis of nicotine.Methods The in-vitro recovery of nicotine was detected by concentration difference method(increment method and decrement method).The stability of recovery was observed.The effects of the pretreatment with perfusate,and the flow rates and concentrations of the perfusate on nicotine recovery were studied.Results Filtering and stirring treatment increased the recovery,but the time of ultrasound degassing showed no effect on the recovery.The recovery detected by increment method was as the same as that detected by decrement method.Nicotine recovery was independent of nicotine concentration in the external medium,showing good stability.Conclusion It is necessary to filter and degas the perfusate before microdialysis.Retrodialysis can be used for the determination of in-vivo nicotine recovery.
