Determination of Berberine Hydrochloride in Shaoyao Decoction by HPLC
- VernacularTitle:高效液相色谱法测定芍药汤中盐酸小檗碱的含量
- Author:
Lin CHEN
;
Limei LIU
;
Ruihai WANG
- Publication Type:Journal Article
- Keywords:
Shaoyao decoction;
berberine hydrochloride;
HPLC;
content determination [1]
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of berberine hydrochloride in Shaoyao decoction. Methods HPLC was performed on an Agilent XDB C18 column by using acetonitrile-0.05 mol/L potassium dihydrogen phosphate-triethylamine (30∶70∶0.14) as mobile phase at a flow rate of 1.0 mL/min, the column temperature was 25 ℃ and the UV detection wavelength was 345 nm. Results Berberine hydrochloride showed a good linearity redationship in the range of 0.07～1.40 ?g. The average recovery was 98.63% (n=6, RSD=1.21%). The average content was 1.55%. Conclusion The method is simple and accurate, with good repeatability, and can be used for the quality control of Shaoyao decoction.
