Determination of Complanatuside in Semen Astragali Complanati and Its Processed Products by HPLC
- VernacularTitle:高效液相色谱法测定沙苑子及其炮制品中沙苑子苷的含量
- Author:
Cheng HE
;
Xiaojing TANG
;
Yuwu CHEN
- Publication Type:Journal Article
- Keywords:
Semen Astragali Complanati;
complanatuside;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for the determination of complanatuside in Semen Astragali Complanati, and study the content change of complanatuside processed. Method HPLC was used with Apollo-C18 column (4.6 mm?250 mm, 5 ?m), acetonitrile-0.1%phosphoric acid (21∶79) as mobile phase, the flow rate at 1.0 mL/min, and detected wavelength was set at 266 nm. Result Complanatuside showed a good linearity relationship in the range of 0.041 6～0.665 6 ?g, r =0.999 9. The average recovery was 100.6%, RSD was 0.73%. Conclusion The method is simple, accurate, reproducibility and strong specificity. It can be used for the quality control of Semen Astragali Complanati. Processing with salt can lead to content decrease of complanatuside in Semen Astragali Complanati.
