Transformation of Glycyrrhiza uralensis genomic DNA into yeast mediated by ion implantation
- VernacularTitle:离子注入介导甘草基因组DNA转化酵母菌
- Author:
Xiang JIN
;
Peihong MAO
;
Jie LU
- Publication Type:Journal Article
- Keywords:
ion implantaton;
Glycyrrhiza uralensis F. genome DNA;
recombined yeast;
glycyrrhizic acid
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective With the sole object of glycyrrhizic acid products,the methods were investigated for Glycyrrhiza uralensis genomic DNA transformation into Hansenula anomala by nitrogen and argon ion implantation.Methods The genomic DNA from G.uralensis was randomly transferred into H.anomala by nitrogen and argon ion bombardment.The recombined yeasts were cultured by the slant and liquid cultivation,in which the contents of glycyrrhizic acid and glycyrrhetic acid were determined by acetic anhydried-H2SO4 qualitative test and RP-HPLC determination.Results Five recombined yeast strains that produced glycyrrhizic acid and glycyrrhetic acid were obtained.After cultured in liquid medium for 96 h,the highest content of glycyrrhizic acid in the cultivation liquid was 114.49 mg/L and that of 18?-glycyrrhetic acid and 18?-glycyrrhetic acid were respectively 0.56 and 0.81 mg/L by RP-HPLC.A kind of unknown red component was found in the cultivation liquid of one recombined strain by TLC.Conclusion The recombined yeast strains of producing glycyrrhizic acid could be obtained G.uralensis genomic DNA transformation into yeast mediated by the ion implantation.
