Cell suspension culture of Gentiana macrophylla(Ⅰ)
- VernacularTitle:秦艽细胞悬浮培养研究(Ⅰ)
- Author:
Xiangjun QI
;
Ruyi CHEN
;
Wei WANG
- Publication Type:Journal Article
- Keywords:
Gentiana macrophylla Pall.;
gentiopicroside;
suspension culture;
culture condition
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish cell suspension culture system of Gentiana macrophylla.Methods Good cell line was selected by the methods of small cell aggregate and the effects of different media,inoculation age of cells,initial pH value,culture temperature,and rotation speed on cell growth and gentiopicroside accumulation were investigated.Results The eligible cell suspension culture conditions were:MS media as the basic medium,cells with inoculation age of 15 d,initial pH 7,and rotation speed of 110 r/min at 25 ℃,culturing in Erlenmeyer flasks.Conclusion Optimal cell suspension culture system of G.macrophylla has been established by shake flasks.The different effects of media on cell growth and gentiopicroside accumulation indicate that some inorganic ions have significant effects on regulation.
