Proteome analysis on differential expression of liver proteins from Oncomlania hupensis induced by Eomecon chionanthe alkaloids
- VernacularTitle:血水草生物碱致钉螺肝脏蛋白质差异表达分析
- Author:
Ling PENG
;
Qiongyao HUANG
;
Jianjun LIU
;
Fei PENG
;
Hui SUN
;
Shishan YUAN
- Publication Type:Journal Article
- Keywords:
Eomecon chionanthe Hance;
alkaloids;
Oncomlania hupensis;
liver;
proteomics
- From:
Chinese Traditional and Herbal Drugs
1994;0(03):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To analyze the influence of Eomecon chionanthe alkaloids(ECA)on differential expression of liver proteins from Oncomlania hupensis.Methods O.hupensis was immersed in 10 mg/L ECA or clean water for 36 h and livers were isolated from live snails.Total liver proteins were extracted and separated by two-dimensional gel electrophoresis,differentially expressed proteins between ECA group and clean water group were selected and analyzed by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry(MALDI-TOF-MS)and tandem mass spectrometry sequencing of tryptic peptides.Results There were(354?110)and(311?12)spots observed in ECA group and in water group,respectively.Eleven differential expressed proteins were identified and all were up-regulated in ECA group.The eleven identified proteins were hypothetical protein,ENSANGP00000022175,class I aldehyde dehydrogenase,unknown protein,ENSANGP00000027067,protein similar to ankyrin 3 isoform 1 isoform 4,protein similar to hepatitis A virus cellular receptor 1 short form,mitochondrial isocitrate dehydrogenase 2-like,protein similar to keratin,keratin-10,and keratin-1,respectively.Conclusion Expression of liver proteins from O.hupensis could be changed when immerse in ECA.
