Comparison between Different Methods and Reagents for Testing Anti-HCV in Blood Donors
- VernacularTitle:用不同方法和试剂检测献血者抗-HCV的比较
- Author:
Li LAI
- Publication Type:Journal Article
- Keywords:
ELISA, PHA, RIBA, Anti-HCV
- From:
Chinese Journal of Blood Transfusion
1988;0(04):-
- CountryChina
- Language:Chinese
-
Abstract:
82 serum samples from the blood donors were comparatively tested by using different reagent kits and two methods (enzyme-linked immunosorbent assay, ELISA anl passive hemagglutination inhibition assay, PHA). The results are as follows: the positive rates of A1, A2 , A3 , A4-1 and B (containing mixed antigens) are 56.09%, 71.95%, 47.56%, 63.41% and 59.75% respectively; and the positive rates of A4-2 and A4-3 (containing single antigens) are 41.46% and 62.19% respectively. The results of testing 8 of all the serum samples by four-gene-recombinent immunoblot assay (RIBA) confirmed that the results of A1, A2, A3, A4-1 and A4-3 were consistent with them. The 76th sample with positive result when tested by RIBA had a negative result when tested by RIBA, while the 82th sample with negative result when tested by RIBA had posi tive one when tested by B. It is suggested that reagent kits made in China left much to be desired in antigen-matching, compactness and cut-off value determining, though they could screen out most of the donors with positive anti-HCV results.
