USING SNAIL ENZYMES TO EXTRACT AND PURIFY THE DNA OF CRYPTOCOCCUS
- VernacularTitle:用蜗牛酶提取和提纯隐球菌DNA
- Author:
Zhiqiang ZHAO
- Publication Type:Journal Article
- Keywords:
Cryptococcus;
DNA;
Extraction;
Snail enzyme
- From:
Microbiology
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
This article introduced a method to extrct and purify the DNA of Cryptococcus. Protoplasts were obtained after digested by snail enzyme. We washed them with SE liquid again and again to get rid of the capsular polysaccharides and split them with thermal treatment of 55 degrees centigrade. Then, satisfactory results of the extrction and the purification were achieved. From one wet gram of the yeast, more than 600 ?g DNA can be acquired. The DNA gained is especially suited for the determination of G+C mol% content by use of the T_m method.
