Triglyceride level changes and potential mechanism on alcoholic fatty liver cell model induced in vitro:with or without diammonium glycyrrhizinate
- VernacularTitle:甘利欣作用前后体外酒精性脂肪肝细胞模型甘油三酯水平变化及其可能作用机制
- Author:
Longhui LI
- Publication Type:Journal Article
- Keywords:
Diammonium glycyrrhizinate injection;
Triglyceride level;
Alcoholic fatty liver
- From:
Journal of Chongqing Medical University
1986;0(02):-
- CountryChina
- Language:Chinese
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Abstract:
Objective:To study the triglyceride level changes with or without Diammounium Glycyrrhizinate(DIG)and the potential mechanism on alcoholic fatty liver cell model induced in vitro.Methods:MTT assay was used to screen the optimal concentration of ethanol and DIG injection.Ultra-microstructure was observed under transmission electron microscope.Intracellular triglyceride concentration was measured by hol-automatic biochemistry.Expressions of peroxisome proliferator-activated receptor-gamma (PPAR?),sterol regulatory element binding protein-1(SREBP-1)and SREBP cleavage activating protein(SCAP)were determined by immunocyto-chemistry(ICC).Results:1.The screened optimal DIG concentration was 2.273?g/ml.2.Masses of lipid droplets in cytoplasm were observed under transmission electron microscope.3.Hol-automatic biochemistry measured intracellular triglyceride concentration to be(1.84?0.16)mmol/L and(1.05?0.12)mmol/L respectively in Ld30 group and DIG group,and the difference was statistically significant(P
