Effect of Electroacupuncture on the Expression of Hippocampal Calbindin-D28K in Hyperlipemia Rats with Concurrent Cerebral Ischemia
- VernacularTitle:电针对高脂血症合并脑缺血损伤大鼠海马区钙结合蛋白D28K蛋白表达的影响
- Author:
Huifang MA
;
Xiujun REN
;
Ya TU
;
Lei ZHOU
- Publication Type:Journal Article
- Keywords:
Hyperlipemia with cerebral ischemia;
Electroacupuncture;
Calbindin D28K expression;
Hippocampal CA 3 area
- From:
Acupuncture Research
2010;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effect of electroacupuncture(EA) on Calbindin D28K(CB) expression and apoptosis in hippocampus in hyperlipemia rats with concomitant cerebral ischemia(CI).Methods Forty male SD rats were rando-mized into control,hyperlipemia+CI(model),acupuncture Ⅰ,and acupuncture Ⅱ groups,with 10 cases in each.Hyperlipemia model was established by feeding the animals with high fat forage for 6 weeks and CI model established by occlusion of the unila-teral middle cerebral artery.For rats of acupuncture Ⅰ group,EA(1-3 mA,15 Hz) was applied to bilateral "Sanyinjiao"(SP 6) and "Fenglong"(ST 40) for 20 min every time,once daily for 10 days before CI.For rats of both acupuncture Ⅰ and Ⅱ groups,after CI,EA was applied to SP 6,ST 40,"Baihui"(GV 20) and "Shuigou"(GV 26) were punctured with filiform needles and stimulated by twirled the needle with hand continuously for 1 min,once daily for 7 days.The apoptotic cells of hippocampal CA 3 area were displayed by TUNEL(terminal deoxynucleotidyl transferase biotin-dUTP nick end labeling) method.CB antibody staining with immunohistochemistry was used to show CB-positive cells which were observed by optic microscope.Results In comparison with control group,the percentage of apoptotic cells of the hippocampal CA 3 area in model group increased considerably(P
