Determination of tetrahydropalmatine and rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid by HPLC
- VernacularTitle:HPLC测定安神养血口服液中3种生物碱延胡索乙素、钩藤碱和异钩藤碱含量
- Author:
Jie WANG
;
Yu LONG
;
Aiju JIAO
;
Zonghe LIU
;
Lina XIN
- Publication Type:Journal Article
- Keywords:
tetrahydropalmatine;
rhynchophylline;
isorhynchophylline;
Stephania kwangsiansis;
Uncaria rhynchophylla(Miq.)Jacks.;
Anshen Yangxue Oral Liquid;
HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM: To establish the method for determining tetrahydropalmatine、rhynchophylline and isorhynchophylline in AnshenYangxue Oral Liquid(Ramulus uncariae cum uncis, stephania kwangsiansis,Radix polygoni multiflori praeparata cum succo glycinus sotae,Caulis Polygoni multiflori and Pine needle). METHODS: Tetrahydropalmatine、rhynchophylline and isorhynchophylline were determined by HPLC.Chromatographic condition was composed of Kromasil C_18 column,a mixture of methanol and water(55∶45) as mobile phase with 0.01 mol/L triehthylamine,adjusted with acetic acid to pH of 7.5,UV detection wavelength of rhynchophylline and isorhynchophylline was set at 254 nm,UV detection wavelength of tetrahydropalmatine was set at 281 nm. RESULTS: The averagere recoveries of tetrahydropalmatine、rhynchophylline and isorhynchophylline were 98.47%、99.04%and 98.75% respectively;RSD were 0.95%、2.6%and 1.6%,respectively. CONCLUSION: This method is simple,sensitive,accurate,and can be used for determining tetrahydropalmatine、rhynchophylline and isorhynchophylline in Anshen Yangxue Oral Liquid.
