Effects of Ginsenoside Rg_1 on the Proliferation of Neural Stem Cells Cultured in Vitro
- VernacularTitle:人参皂苷Rg_1对体外培养胚胎神经干细胞增殖作用的影响
- Author:
Zhihuan ZHOU
;
Xiuyun WANG
;
Peiru ZHONG
- Publication Type:Journal Article
- Keywords:
ginsenoside Rg1;
embryonic neural stem cells;
proliferation
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To explore the effect of ginsenoside Rg1 on proliferation of neural stem cells (NSCs) in vitro. Methods The NSCs cultures were generated from the brain of embryonic day 16 SD rat. Primitive NSCs were cultured, proliferated and passaged. The NSCs were identified by the immunocytochemical (ICC) staining of Nestin. The ICC staining of BrdU was adopted to characterize the proliferation of NSCs. According to limited dilution method, the effect of ginsenoside Rg1 on the proliferation of NSCs was observed. Results The NSCs were successfully cultured and proliferated in vitro. Different dosages (100, 4, 8 ?mol/L) of ginsenoside Rg1 could promote the proliferation of NSCs in vitro. Compared with the control group, the numbers of nerurospheres of the three dosage ginsenoside Rg1 groups (40, 4, 0.4 ?mol/L) were increased obviously. Conclusions Ginsenoside Rg1 significantly promote the proliferation of NSCs in vitro.
