Seropharmacological Effect of Tujian Mixture on Phospho-Akt and PTEN of Glomerular Mesangial Cell Cultured in High Concentrations of Glucose
- VernacularTitle:菟箭合剂含药血清对高糖条件培养的肾小球系膜细胞磷酸化Akt和PTEN的作用
- Author:
Dehai YIN
;
Xiaochun LIANG
;
Hong ZHANG
- Publication Type:Journal Article
- Keywords:
Tujian Mixture;
glomerular mesangial cell;
diabetic nephropathy;
PI3K-Akt signaling pathyway;
PTEN
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To observe the effects of serum containing the medicine of Tujian Mixture on phospho-Akt and phospho-PTEN of glomerular mesangial cell (MC) cultured in high concentrations of glucose. Method The glomerular mesangial cells from SD rat were divided into six groups:the normal control group, the group of MC cultured in high concentrations of glucose, and four other groups cultured in high concentrations of glucose + different concentrations of rat’s serum containing Tujian Mixture. After 72 hours, the content level of phospho-Akt (Thr308) and phospho-PTEN (Ser380) in MC was detected by using sandwich ELISA. Results The level of phospho-Akt (Thr308) in the group of MC cultured in high concentrations of glucose was significant higher than that in the normal control group (P
