Extraction and Content Determination of Polysaccharide in Imperata Cylindrica
- VernacularTitle:白茅根多糖的提取与含量测定
- Author:
Haixia WANG
;
Yun WU
;
Weijing SHI
- Publication Type:Journal Article
- Keywords:
Imperata Cylindrica;
polysaccharide;
orthogonal design;
phenol-dense sulphuric acid method;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To optimize the extracting conditions of polysaccharide from Imperata Cylindrica and establish a suitable method for its content determination. Methods Based on the qualitative yield of polysaccharide extraction, a orthogonal test was employed to evaluate the effect of four factors including extracting temperature, extracting time, the extraction times and the ratio of material to liquid. The phenol-dense sulphuric acid method was applied for the content determination of polysaccharide. Results The times of extraction and the extracting temperature influenced the content of polysaccharides mostly, while the duration of extraction and the amount of water showed little influence. Conclusions The optimum extraction condition was refluxing and extracting for 3 times at 85 ℃ for 3 hours, and with 15 folds of water for every time. The contents of polysaccharide in Imperata Cylindrica collected from two different places were above 0.15%.
