Activated Caspase-8 Regulates Bcl-2 Expression in Fas-and Actinomycin D-induced Apoptosis in Bel-7402 Cells
- VernacularTitle:在Fas和放线菌素D诱导Bel-7402细胞凋亡中激活的caspase-8调节Bcl-2表达
- Author:
Lu WANG
;
Yu WANG
;
Chunhui XIA
- Publication Type:Journal Article
- Keywords:
Fas;
caspase-8;
Bcl-2;
cell apoptosis
- From:Space Medicine & Medical Engineering
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate whether caspase-8 regulates Bcl-2 expression in Fas and Actinomycin D(AD) induced apoptosis in Bel-7402 cells.Methods Bel-7402 cells were divided into four groups randomly,that is:control,Fas group,Fas-AD group and Ac-IEFD-cho group,and treated with Fas-AD in the presence/absence of caspase-8 inhibitor Ac-IEFD-cho.The expressions of caspase-8 and Bcl-2 were determined with Western Blot and RT-PCR,respectively.Results Compared with control group and Fas group,after Bel-7402 cells were treated with Fas-AD,typical apoptosis morphology was clearly observed,the expression of activated caspase-8 increased significantly,and the Bcl-2 expression decreased apparently.However,after Bel-7402 cells were treated with Fas-AD in the presence of the caspase-8 inhibitor Ac-IEFD-cho,the expressions of activated caspase-8 decreased while the Bcl-2 expression increased.Conclusion Activated caspase-8 can regulate Bcl-2 in Fas-and AD-induced apoptosis in Bel-7402 cells.
