Expression of ? Defensin Genes in Rat Airway Epithelium after Intraperitoneal Injection of Emulsified Isoflurane
- VernacularTitle:腹腔注射乳化异氟醚对大鼠气道上皮组织?防御素mRNA表达的影响
- Author:
Lin LUO
;
Luxia DENG
;
Ning HUANG
;
Tao ZHU
- Publication Type:Journal Article
- Keywords:
emulsified isoflurane;
defensin;
TNF;
IL
- From:Space Medicine & Medical Engineering
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To examine the expression of ? defensin genes and inflammatory cytokines genes in rat airway epithelium after intraperitoneal injection of emulsified isoflurane.Methods Thirty-six SD rats were assigned into six groups(A-F) randomly.The tissue from rat airway epithelium were harvested at 1 h after intraperitoneal injection of normal saline,30% fat milk and 8% emulsified isofluranes of 4 different dosages(0.25,0.5,1.0 and 1.5 times of ED50).Then expressions of ? defensin(BD-1,BD-2),tumor necrosis factor-alpha(TNF-?),interleukin beta(IL-1?),IL-8 mRNA were measured by semiquantitative polymerase chain reaction(PCR) using GAPDH as an internal standard.Results Except BD-1 and IL-8 mRNA,marked increases in expressions of TNF-?,IL-1? and BD-2 genes were observed with a dosage-dependent manner.Conclusion The expressions of BD-2 genes and inflammatory cytokines genes(IL-1?,TNF-?)in rat airway epithelium increase significantly in a dosage-dependent manner after intraperitoneal injection of emulsified isoflurane.
