Purification technology of iridoid glycosides and xanthones from Swertia mussotii by macroporous resin
- VernacularTitle:大孔树脂分离纯化川西獐牙菜中环烯醚萜苷类和酮类成分的工艺研究
- Author:
Lina MA
;
Tiejun ZHANG
;
Chengwang TIAN
;
Shiliu HAN
;
Qiang ZHAO
- Publication Type:Journal Article
- Keywords:
Swertia mussotii Franch.;
iridoid glycosides;
xanthones;
macroporous resin
- From:
Chinese Traditional and Herbal Drugs
1994;0(02):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the separation and purification technology of iridoid glycosides and xanthones from Swertia mussotii by macroporous resin.Methods The dynamic adsorption and desorption characteristics of macroporous adsorption resins HPD-300,HPD-400,HPD-600,AB-8,DM-301,and D-101-Ⅰ for swertiamarin and swertianolin were investigated.On the base of the investigation,the better macroporous adsorption resin was chosen to finish the experiments.Results The adsorption and elution characteristics of HPD-300 used to separate and purify iridoid glycosides and xanthones were very good.In the course of adsorption,the amount of used adsorption is 0.9 g dried medicinal herb/mL resin,with about 8 pH value of adsorption solution,and the volumetric rate is 2 BV/h; In the course of elution,the resin column chromatography was eluted gradiently with 7 BV 20% and 5 BV 70% EtOH after removing impurities with water.The elution rates were both more than 90%,and the contents of iridoid glycosides and xanthones were both up to 50% in the two dried fractions of 20% and 70% ethanolic elutions.Conclusion HPD-300 is an ideal resin with the best enrichment for separating and purifying both iridoid glucosioles and xanthanoes simultanously.
