Up-regulation of tumor necrosis factor-? on c-jun in cultured human retinal pigment epithelial cells
- VernacularTitle:肿瘤坏死因子上调人视网膜色素上皮细胞c-jun的表达
- Author:
Jingbo WANG
- Publication Type:Journal Article
- Keywords:
Retinal pigment epithelium;
Tumor necrosisfactor;
c-jun
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To investigate the effect oftumor necrosis factor (TNF)-? on the expression ofc-jun in the cultured human retinal pigment epithelial(RPE)cells.Methods:RPE cells were exposed to 40 ng/ml TNF-? for 0,10,20,40 and 40 minutes,respectively.Immunofluorescence staining and reverse transcription polymerase chain reaction(RT-PCR)were used to detect the levels of c-jun protein and mRNA in RPE cells.Results:Untreated RPE cells revealed faint or none green fluorescence of c-jun in the cytoplasm and some nuclei.With exposure to TNF-?,c-jun increased obviously with stronger green fluorescence.With treatment of TNF-?,c-jun mRNA increased gradually and enhanced to the peak at 40 min.Conclusion:TNF-? can up-regulate c-jun protein and mRNA in cultured human RPE cells and induce its nuclear transposition,which suggests the activation of c-jun.
