Establishment and drug-resistance gene expression of cisplatin-resistant cell line Acc-3/CDDP
- VernacularTitle:多药耐药口腔腺样囊性癌细胞株(Acc-3/CDDP)的建立及其细胞中相关耐药基因表达的变化
- Author:
Fujun ZHANG
- Publication Type:Journal Article
- Keywords:
Oralcavity;
Adenoid cystic carcinoma cell;
Multi-drug resistance;
Gene
- From:
Journal of Chongqing Medical University
1987;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective:To establish a CDDP-resistant cell line from human oral adenoid cystic carcinoma cell line Acc-3 and compare the drug-resistance gene expression between them.Method:The CDDP-resistant cell line Acc-3/CDDP was established by increasing the concentration of CDDP added to Acc-3 cells step wisely.The index of drug-resistance was estimated by MTT assay.The expression of MDR-1,MRP-1,GST-?and Bcl-2 were measured byRT-PCR optical densityOD.Result:The resistance factor(sRF=IC5(0Acc-3/CDDP) /IC5(0Acc-3))for CDDP,5-Fu,VCR,MTX and PYMwere 8.9,1.97,2.83,2.42,1.1 respectively.The expression of drug-resistance gene MRP-1,GST-?and Bcl-2 increased in Acc-3/CDDP cells.MDR-1 was expressed in Acc-3/CDDP,but none in Acc-3.Conclusion:MDR-1,MRP-1,GST-?and Bcl-2 were significantly over expressed in CDDP-resistant cell line Acc-3/CDDP,which can provide effective models for the study on multi-drug resistance of oral carcinoma.
