HPLC Fingerprint Analysis of Ganzhixiao Decoction
- VernacularTitle:肝脂消汤剂的HPLC指纹图谱研究
- Author:
Lan MA
;
Tongfang DU
;
Gang WEI
;
Cuiling LIU
;
Yuechun HUANG
- Publication Type:Journal Article
- Keywords:
Ganzhixiao Decoction;
Fingerprint;
HPLC
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a fingerprint analysis method for Ganzhixiao Decoction. Methods RP-HPLC was applied with Phenomenex Synergi 4?Hydro-RP 80A as the chromatic column,methanol-0.1 %H3PO4 solution (gradient elution) as the mobile phase,detection wavelength at 280 nm,flow rate being 1.0 mL/min,and column temperature at 30 ℃. Results Twenty common peaks were presented in 10 batches of Ganzhixiao Decoction and the positions of 20 characteristic peaks were identified,including 5-hydroxymethyl furfural,chlorogeni C acid,Salvianolic acid B. Conclusion The method is reliable and accurate,and can provide a reference for the research of Ganzhixiao Decoction.
