Research on Fingerprint of Radix Dipsaci by HPLC
- VernacularTitle:续断药材指纹图谱的研究
- Author:
Yue CAO
;
Wei PENG
;
Weiwei SU
;
Songping LUO
- Publication Type:Journal Article
- Keywords:
Radix Dipsaci;
HPLC;
Fingerprint
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish chromatographic fingerprint of water and ethanol soluble component of Radix Dipsaci by using HPLC for identifying and evaluating its quality. Methods HPLC method was set up with Agilent HC-C18 column. The mobile phase was comprised of acetonitrile(A) and 0.05mol/L KH2PO4-H3PO4 buffer solution pH=3.0(B) in gradient elution mode. UV detection wavelength was at 220 nm,with a flow rate of 1.0 mL/min. Results Ten batches of Radix Dipsaci samples from different habitats and harvested in different times were analyzed. HPLC fingerprint of medicinal material of Radix Dipsaci was established preliminarily. Seventeen characteristic peaks were confirmed and the fingerprints of different samples were compared by similarity evaluation software. Conclusion This method shows high precision and good repeatability,so it can be used to assess the quality of Radix Dipsaci.
