Determination of Hyperoside in Jintu Liangguang Capsules by RP-HPLC
- VernacularTitle:RP-HPLC法测定金菟亮光胶囊中金丝桃苷的含量
- Author:
Gang WANG
;
Yingbiao TIAN
;
Songsong YANG
- Publication Type:Journal Article
- Keywords:
Jintu Liangguang Capsules;
Hyperoside;
RP-HPLC;
Content determination
- From:
Traditional Chinese Drug Research & Clinical Pharmacology
1993;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a determination method for the hyperoside in Jintu Liangguang Capsules. Methods Reverse-phase HPLC with Dikma Diamons C18 column was applied. The chromatographic conditions were as follows:methanol-0.025 mol/L of thophosphoric acid (40 ∶60) as the mobile phase,detection wavelength at 370 nm,column temperature set at 40 ℃and the flow rate being 0.8 mL/min. Results The linear range of calibration curve was 0.303 ～1.818 ug/uL for the hyperoside (r=0.999 6). The average recovery was 95.2 %and RSD was 1.3 %. Conclusion The method is simple and accurate,and can be used for quantitative analysis of Jintu Liangguang Capsules.
