Optimization of Macroporous Resin for Isolation and Purification of Total Alkaloids from Rhizoma Coptidis
- VernacularTitle:大孔树脂分离纯化黄连总生物碱型号的筛选
- Author:
Ying ZHANG
;
Weimin LI
;
Ying GAO
- Publication Type:Journal Article
- Keywords:
RHIZOMA COPTIDIS/ isolation & purification;
TOTAL ALKALOIDS/analysis;
BERBERINE HYDROCHLORIDE /analysis;
MACROPOROUS RESIN
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To select a best type of macroporous resin for the isolation and purification of total alkaloids from Rhizoma Coptidis.Methods With the adsorption rate and desorption rate of total alkaloids from Rhizoma Coptidis as the observation indexes,an initial selection of macroporous resin was carried out in AB-8,HP20,LD605,ADS-3,ADS-5,D151,DA-201,XAD7 and NKA-9.After the initial selection,the desorption rate of berberine hydrochloride was used as the index for the further selection.Results Of the nine types of resin,ADS-3 had good adsorption and desorption activities,with an adsorption rate being 97.26% and a desorption rate being 84.82%.Meanwhile,the amount of desorpted berberine hydrochloride was up to 9.311 mg.Conclusion ADS-3 shows good adsorption and desorption properties for total alkaloids and berberine hydrochloride,and can be used for the isolation and purification of total alkaloids from Rhizoma Coptidis.
