Effect of Angong Niuhuang Bolus on High Mobility Group Box-1 Protein Expression and Myeloperoxidase Activity in Lung of Sepsis Rats
- VernacularTitle:安宫牛黄丸对脓毒症大鼠肺组织高迁移率族蛋白B1基因表达及髓过氧化物酶活性的影响
- Author:
Dan ZHANG
;
Zhiyi HU
;
Ping HUANG
;
Jun LI
;
Yanli WANG
- Publication Type:Journal Article
- Keywords:
ANGONG NIUHUANG BOLUS/pharmacology;
SEPSIS/TCD therapy;
GENE EXPRESSION REGULATION;
DISEASE MODELS,ANIMAL;
RATS
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effect of Angong Niuhuang Bolus(ANB) on mRNA expression of high mobility group box-1(HMGB1) protein and myeloperoxidase activity in the lung of rats with sepsis induced by cecal ligation and puncture(CLP) method.Methods Seventy-five male SD rats were randomly divided into normal control group,model group,AG490(a Janusk kinase inhibitor) treatment group(subcutaneous injection of AG490 8mg/kg 0.5 h before CLP),rapamycin(RPM,a signal transduction and transcription activator inhibitor) treatment group(subcutaneous injection of RPM 0.4 mg/kg 0.5 h before CLP),and low-,middle-and high-dose ANB groups(gastric gavage of ANB in the dose of 1.0,2.0 and 3.0 g?kg-1?d-1 respectively).The animals were sacrificed 24h after CLP,and then lung tissue samples were collected to determine the expression of HMGB1 mRNA by the method of reverse transcription polymerase chain reaction(RT-PCR).Meanwhile,the activity of pulmonary myeloperoxidase(MPO) was also measured.Results Compared with the normal control group,the mRNA expression of HMGB1 was significantly enhanced(P
