Effects of Compound Ganduqing Decoction on Activation and Apoptosis of Iron-overloading Hepatic Stellate Cells
- VernacularTitle:复方肝毒清对铁超载肝星状细胞活化和凋亡的影响
- Author:
Yuan JIANG
;
Ling ZHANG
;
Jinyang HE
;
Xingbo GUO
- Publication Type:Journal Article
- Keywords:
LIVER FIBROSIS/TCD therapy;
COMPOUND GANDUQING DECOCTION /pharmacology;
HEPATIC STELLATE CELLS/pathology;
HEPATIC STELLATE CELLS/ultrastructure;
CELL CULTURE
- From:
Journal of Guangzhou University of Traditional Chinese Medicine
2001;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To investigate the effects of Compound Ganduqing Decoction(CGD) on activation and apoptosis of iron overloading hepatic stellate cells(HSC).Methods The cultured HSC-T6 cells were used for experimental cells,and were divided into normal control group,model group and CGD treatment group(in the dose of 0.08g/L).HSC model of iron overloading was induced by incubation with ammonium iron citrate.Immunohistochemical assay was used for the detection of alpha smooth muscle actin(?-SMA) expression.Quantitative polymerase chain reaction(PCR) was applied for the detection of transforming growth factor beta 1(TGF-?1) mRNA expression.TUNEL technique was used for the examination of apoptosis of HSC-T6.Electron microscope was used for the observation of ultrastructure of HSC-T6.Results In the normal control group and the model group,there showed large amount of ?-SMA expression in HSC-T6,but little apoptosis.However,in CGD group ?-SMA expression was decreased obviously,TGF-?1 mRNA expression was reduced,and apoptosis of HSC-T6 was obvious(P
