Simultaneous determination of rutin and naringenin-7-o-glucoside in extraction of Lysimachia clethroide Duby by HPLC
- VernacularTitle:HPLC同时测定珍珠菜提取物中芦丁和柚皮素-7-O-葡萄糖苷的含量
- Author:
Haimin PAN
;
Bengang YOU
;
Lihua TANG
;
Xinzhang LI
;
Shilin YANG
- Publication Type:Journal Article
- Keywords:
HPLC;
Lysimachia clethroide Duby;
rutin;
naringenin-7-o-glucoside
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a HPLC method for simultaneous determination of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby.METHODS:Using Diamonsil C_ 18(4.6 mm ?250 mm,5?m)as analytical column.The mobile phase consisted of acetonitrile(A)and 0.1% phosphoric acid(B)with gradient elution:0~18 min,83%~80%B;18~30 min,80%~86%B.The detection wavelength of rutin and naringenin-7-o-glucoside was at 254 nm and 281 nm,respectively.The flow rate was 1.0 mL/min;Column temperature was at 35 ℃.RESULTS :A better separating effect was obtained with the HPLC gradient elution method.The linear calibration curve of rutin and naringenin-7-o-glucoside were obtained in the concentration range of 1.00~48.00 ?g/mL(r=0.999 3)and 0.64~40.72 ?g/mL(r=0.999 8),respectively.CONCLUSION:The HPLC method is accurate,simple and can be used to determine the contents of rutin and naringenin-7-o-glucoside in Lysimachia clethroide Duby simultaneously.
