Simultaneous determination of two compounds in Mosla chinensis by RP-HPLC
- VernacularTitle:RP-HPLC同时测定不同采收期江香薷两种成分的含量
- Author:
Haowu HU
;
Xiaoming XIE
;
Rengeng SHU
;
Yuesheng WANG
- Publication Type:Journal Article
- Keywords:
Mosla chinensis Maxium;
luteolin;
negletein;
RP-HPLC
- From:
Chinese Traditional Patent Medicine
1992;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To establish a method for simultaneously determining luteolin and negletein in Mosla chinensis Maxium collected in picking times.METHODS:The Angilent Zorbax,Extend C-18 column(4.6 mm?250 mm,5 ?m)was used with methanol-water(containing 0.4% phosphonic acid)(50 ∶50)as mobile phase.The flow rate was 1.0 mL/min.The detection wavelength was set at 350 nm.RESULTS:The method had the good linear relationship within the range of 0.04-0.61 ?g for luteolin and within the range of 0.20-3.01 ?g for negletein,respectively,with the correlation coefficient of 0.999 9.The average recoveries of the two flavones were 97.32%,98.60%,respectively.The contents of luteolin and negletein in Mosla chinensis Maxium.varied in different picking times,which reached the its maximum at florescence(264.8 ?g/g,2 746.3 ?g/g)in August,respectively.CONCLUSION:The method is fast,rapid,convenient,accurate and can be used for quality control of Mosla chinensis Maxium.
