Determination of Rhamnocitrin in Oxytropis falcate Bunge by HPLC
- VernacularTitle:HPLC法测定藏药镰形棘豆中鼠李柠檬素的含量
- Author:
Hua JIANG
;
Junru HU
;
Xia LIU
- Publication Type:Journal Article
- Keywords:
Oxytropis falcate Bunge;
rhamnocitrin;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method of HPLC for determination of rhamnocitrin in Oxytropis falcate Bunge.Methods The HPLC method was used.Chromatographic column:Lichrospher C18(4.6 mm? 250 mm,5 ?m) reversed-phase column;mobile phase:0.4% phosphoric acid in water-methanol(20∶80);flow rate:1.0 mL/min;detective wavelength:350 nm.Results Rhamnocitrin was liner at the range of 4～ 30 ?g(r =0.999 8),and its average recovery rate was 99.7%,RSD=1.4%.Conclusion The method was simple and accurate,so it can be used for the quality control of Oxytropis falcate Bunge.
