Determination of Oleanolic Acid in Cortex Aralia Elatae by HPLC
- VernacularTitle:HPLC法测定龙芽楤木药材中齐墩果酸的含量
- Author:
Zhaoyang TAN
;
Hongjia YUAN
- Publication Type:Journal Article
- Keywords:
Cortex Aralia Elatae;
oleanolic acid;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish a method for determination of oleanolic acid in Cortex Aralia Elatae by HPLC.Methods The determination was carried out with Kromasil C18 column(250 mm?4.6 mm,5 ?m),using acetonitrile-water-phosphoric acid(80∶20∶0.42) as the mobile phase and detected at the wavelength of 208 nm.Results Oleanolic acid showed a good linear relationship at the range of 1.164～5.820 ?g,r=0.999 9.The average recovery was 99.70% and RSD was 1.76%(n =5).Conclusion The method was simple and accurate with good reproducibility,and can be used to determine the content of oleanolic acid in Cortex Aralia Elatae.
