Determination of Indirubin in Kangbingan Oral Liquid by Ultraviolet Spectrophotometry
- VernacularTitle:紫外分光光度法测定抗病感口服液中靛玉红的含量
- Author:
Jinhua YIN
;
Shuqian ZHANG
;
Xuemei JIA
- Publication Type:Journal Article
- Keywords:
Kangbingan Oral Liquid;
indirubin;
ultraviolet spectrophotometry;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To determine the content of indirubin in Kangbingan Oral Liquid by UV spectrophotometer methods.Methods The content of indirubin was determined by Heltos Gamma ultravioletvisible spectrophotometer with chloroformic solution of 2～8 ?g/mL confected by standard sample of indigotin and indirubin.The sample of negative blank solution was confected by the prescription without Radix Isatidis.The standard sample and the blank sample were determined separately with the wavelength at 500～700 nm.Results Indirubin had the maximum absorption at wavelength of 540.0 nm and indigotin had the maximum absorption at wavelength of 634.5 nm.The linear correlation was available at the range of 0.20～1.40 ?g/mL,and the correct coefficient was 0.999 4.The average recovery rate was 99.05% and RSD was 0.87%(n=6).Conclusion This method is simple and accurate,and can be used to control the quality of Kangbingan Oral Liquid.
