Induction of dihydroartemisinin on prostate cancer PC-3 apoptosis and its mechanism
- VernacularTitle:双氢青蒿素诱导前列腺癌PC-3细胞凋亡及其机制研究
- Author:
Xiaoling GAO
;
Ziguo LUO
;
Pilong WANG
;
Qingchun LI
- Publication Type:Journal Article
- Keywords:
dihydroartemisinin(DHA);
prostate cancer(PC);
apoptosis
- From:
Chinese Traditional and Herbal Drugs
1994;0(01):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To study the induction of dihydroartemisinin(DHA) on prostate cancer PC-3 apoptosis and its possible mechansim.Methods MTT was employed for cellular viability measurement,flow cytometry(FCM) and transmission electron microscopy(TEM) for observation of apoptosis,and immunocytochemical staining(SP) for analyzing the expression of Bcl-2 and Bax proteins in PC-3 cells treated with DHA of different concentration.Results DHA Significantly inhibited the proliferation of PC-3 cells,induced their apotosis in a time-concentration dependent manner,and led to mitochondrial swelling,nuclear fragmentations and apoptosis body formation,down-expression of Bcl-2 protein,and over-expression of Bax protein correspondence with DHA concentration.Conclusion DHA could induce the apoptosis in PC-3 cells by up-regulating Bax protein and down-regulating Bcl-2 protein.
