Orthoonal exeriment in the preparation technological of ?-linolenicacid liposome
- VernacularTitle:正交实验优选?-亚麻酸脂质体制备工艺
- Author:
Yuegang ZHAO
- Publication Type:Journal Article
- Keywords:
Liposome;
?-linolenic acid;
Orthogonal test
- From:
Journal of Chongqing Medical University
2007;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective :Evening primrose oil contains abundance of Essentia polyunsaturated fatty acids and ?-linolenic acid is the mostly important,but the property of ?-linolenic acid is unstable and easily oxidized,in this experiment the ?-linolenic acid was made into liposome so as to make it stable and more effective,orthogonal test was used to choose the Best Conditions of preparing the liposome of ?-linolenic acid.Methods:The best proportion of phospholipid and evening primrose oil is 0.2:0.1,dissolved in 20 ml chloroform and 40 ml phosphate buffer solution,ultrasonic treatment for 10min.Results:the?-linolenic acid entrapment efficiency was more than 99% and the permeability is low.Conclusion:The method is simple,accurate and reliable and can be used for the new drug development and production.
