Determination of six components in Heiguidou Oil by capillary GC
- VernacularTitle:毛细管气相色谱法同时测定黑鬼豆油中6种成分的含量
- Author:
Lingfei XIANG
;
Runrong ZHANG
;
Haiyan CHEN
;
Xuefeng YE
- Publication Type:Journal Article
- Keywords:
Heiguidou Oil;
assay;
capillary GC
- From:
Chinese Traditional Patent Medicine
1992;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
AIM:To develop a capillary GC method for determining six components in Heiguidou Oil,?-pinene,eucalyptol,borneol,methyl salicylate,cinnamia aldehyde and eugenol. METHODS:GC was applied to quantitative analysis. Chromatographic conditions consisted of PEG-20M(30 m?0.25 mm?0.25 ?m),flame ionization detector(FID),and programmed temperature; initiation temperature was at 80 ℃ for 7 min; the rate of temperature was 15℃/minute,final temperature was at 180 ℃ for 10 min,bypass rate was 50 ∶1. The sample dissolved in absolute alcohol. The external standard was used. RESULTS:The standard curves of conclusion ?-pinene,eucalyptol,borneol,methyl salicylate,cinnamia aldehyde and eugenol were linear within the ranges of 0.322-32.150 mg/mL,0.202 -20.215 mg/mL,0.126-12.630 mg/mL,0.633-63.290 mg/mL,0.174-17.390 mg/mL,0.633-63.290 mg/mL and 0.203-20.255 mg/mL,respectively.RSD were 0.83%,0.48%,0.48%,0.31%,and 0.53 %,respectively. CONCLUSION:The established method is accurate and it can be applied to the simulta-neous assay of six components.
