Determination of Chlorogenic Acid in Reduping Keli by HPLC
- VernacularTitle:高效液相色谱法测定热毒平颗粒中绿原酸的含量
- Author:
Ping LI
- Publication Type:Journal Article
- Keywords:
Reduping Keli;
Chlorogenic acid;
HPLC;
content determination
- From:
Chinese Journal of Information on Traditional Chinese Medicine
2006;0(12):-
- CountryChina
- Language:Chinese
-
Abstract:
Objective To establish an HPLC method for the determination of Chlorogenic acid in Reduping Keli. Methods The determination was performed by HPLC using an YMC ODS-A column (150 mm?4.6 mm, 5 ?m), with mobile phase consisted of acetonitrile-0.2%H_3PO_4 solution (10 : 90) at a flow rate of 1.0 mL/min and a detection wavelength of 327 nm. Results The calibration curve for Chlorogenic acid was linear in the concentration range of 0.0085～0.084 8 mg/mL (r=0.9999). The average recovery was 102.60 % (RSD=1.69%, n =6). Conclusion The method is simple, rapid and applicable for the quality control of Reduping Keli.
